Shrimp White Spot Syndrome - from Pathology to Pathogenomics

Design and Evaluation of a PCR Method for Detecting White Spot Syndrome Virus in Shrimp

Background: White spot syndrome virus (wssv) is the causing agent for white spot disease in shrimp and many crustaceans. This disease is highly contagious and can cause death within 3–10 days under normal culture conditions. Therefore, early diagnosis of the virus is a necessity. Materials and Methods: Primers were designed for three regions of the virus genome and one region of the shrimp geno...

Transcription and identification of an envelope protein gene (p22) from shrimp white spot syndrome virus.

White spot syndrome virus (WSSV) is one of the most virulent pathogens causing high mortality in shrimp. In the present study, an open reading frame (termed the p22 gene) was revealed from a WSSV cDNA library. The gene was expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli and purified. Specific antibody was raised using the purified fusion protein (GST-P22)....

Effects of dietary astaxanthin on the immune response, resistance to white spot syndrome virus and transcription of antioxidant enzyme genes in Pacific white shrimp Litopenaeus vannamei

Three different experiments were conducted to study the effect of dietary astaxanthin on the immune response, resistance to white spot syndrome virus (WSSV) and transcription of antioxidant enzyme genes in Pacific white shrimp Litopenaeus vannamei. Each experiment included one triplicate treatment group (fed with 80 mg/kg astaxanthin supplemented diet) and one triplicate control group (fed with...

Effects of dietary astaxanthin on the immune response, resistance to white spot syndrome virus and transcription of antioxidant enzyme genes in Pacific white shrimp Litopenaeus vannamei

Three different experiments were conducted to study the effect of dietary astaxanthin on the immune response, resistance to white spot syndrome virus (WSSV) and transcription of antioxidant enzyme genes in Pacific white shrimp Litopenaeus vannamei. Each experiment included one triplicate treatment group (fed with 80 mg/kg astaxanthin supplemented diet) and one triplicate control group (fed with...

Antisense RNA mediated protection from white spot syndrome virus (WSSV) infection in Pacific white shrimp Litopenaeus vannamei